Graph pangenome of eggplant and (its) allied species - Unité Génétique et Amélioration des Fruits et Légumes _ INRAE
Conference Poster Year : 2024

Graph pangenome of eggplant and (its) allied species

Björn Usadel

Abstract

Recent advances in sequencing technologies and the reduction of their costs have accelerated the assembly of plant genomes. However single reference genomes do not fully capture the species diversity, leading to the development of the pangenome concept. A pangenome encompasses all DNA sequences available for a species or larger taxa, including single nucleotide polymorphisms (SNPs) and small/large structural variants (SVs). To better characterize these variations, the development of graph-based pangenomes have emerged. They store sequence and SV information in nodes and paths, thereby expanding the linear reference genome's coordinate system to more effectively capture genetic diversity. In this study, Oxford Nanopore long reads from 40 accessions, including 33 accessions of Solanum melongena among which the reference line ‘67/3’, as well as 7 accessions of the allied species S. insanum and S. incanum were assembled using NextDenovo. The resulting assemblies were polished with Medaka and chromosome-scale assemblies were generated for six S. melongena and two accessions each of S. insanum and S. incanum, by incorporating chromosome conformation capture (Hi-C) data. The chromosomes of the other 30 accessions were reconstructed using RagTag, based on the reference line ‘67/3’ sequence. A combination of Helixer (Deep Neural Networks ab initio gene prediction) and Braker-3 (both ab initio and evidence-based gene prediction) was applied to annotate each genome, resulting in a gene set ranging from 30,886 to 33,449, with a BUSCO completeness from 95.3 to 97.2%. The obtained assemblies were used to build up two different graph-based pangenomes. The first one, which includes the 40 accessions, was built using the PanGenome Graph Builder (PGGB) pipeline. The second one, comprising only the 33 S. melongena accessions, was obtained with Minigraph-Cactus pangenome pipeline. The latter was then used for genotyping 311 S. melongena accessions from the EU G2P-SOL project core collection, which were sequenced with Illumina short read sequencing. Overall, 4,532 high-quality SVs were identified. The accession panel was phenotyped, among others, for resistance to fungal wilts at two sites: Montanaso Lombardo (Italy), and Antalya (Türkiye), and associations between phenotype and genotype were identified using BLINK model within GAPIT R package, on multi-environment data. Here, we report the genome-wide association (GWA) results for resistance to Fusarium oxysporum f. sp. melongenae and Verticillium dahliae. Our results highlight that the graph pangenome provides an efficient model for representing and analyzing multiple genomes and their variations. This offers a comprehensive resource for eggplant breeding programs and enhances the understanding of eggplant genetic architecture of traits, particularly in relation to fungal wilt resistance.
Fichier principal
Vignette du fichier
Gaccione_et_al_graph_pangenome_Unito_SIGA_2024_Italy.pdf (236.4 Ko) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-04670168 , version 1 (11-08-2024)

Identifiers

  • HAL Id : hal-04670168 , version 1

Cite

Luciana Gaccione, Marie Bolger, Laura Toppino, Ezio Portis, Sergio Lanteri, et al.. Graph pangenome of eggplant and (its) allied species. 67th annual congress of the Italian Society of Agricultural Genetics, Sep 2024, Bologna, Italy. ⟨hal-04670168⟩
8 View
4 Download

Share

More