Combined in vivo and in silico approaches for predicting the release of bioactive peptides from meat digestion - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Food Chemistry Year : 2018

Combined in vivo and in silico approaches for predicting the release of bioactive peptides from meat digestion

Thierry Sayd
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Christophe Chambon
Caroline Buffière
Didier Remond

Abstract

We studied the kinetics of peptide release during the gastric digestion of meat proteins in vivo, in view to predicting the release of bioactive peptides further on in the digestive tract. Six mini pigs fitted with gastric cannulas received a meal with cooked beef as protein source. Digesta was collected at regular time intervals up to 5½ h. The peptides generated by the gastric digestion of meat were identified and quantified using label-free LC MS, thereafter subjected to in silico digestion mimicking the action of intestinal enzymes. Three clusters of proteins presenting similar evolutions according to their dynamic hydrolysis were obtained. This study clearly improves the in silico prediction of the intestinal release of bioactive peptides by mapping meat protein degradation in the stomach in an in vivo model. Knowledge of the conformation of the peptides released in the stomach further improves this prediction.
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hal-02621622 , version 1 (26-05-2020)

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Thierry Sayd, Claire Dufour, Christophe Chambon, Caroline Buffière, Didier Remond, et al.. Combined in vivo and in silico approaches for predicting the release of bioactive peptides from meat digestion. Food Chemistry, 2018, 249, pp.111-118. ⟨10.1016/j.foodchem.2018.01.013⟩. ⟨hal-02621622⟩
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