Modifications of trout <em>(Oncorhynchus mykiss)</em> muscle proteins by preslaughter activity - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Journal of Agricultural and Food Chemistry Year : 2006

Modifications of trout (Oncorhynchus mykiss) muscle proteins by preslaughter activity

Abstract

The effect of two different preslaughter procedures (limited or 15-min intense muscular activity) on muscle trout proteins was investigated. Muscle was sampled 45 min and 24 h post-mortem, proteins were separated using two-dimensional electrophoresis, and spots of interest were tentatively identified by MALDI-TOF spectrometry. Twenty-nine and 4 spots were differentially represented between the two groups of fish at 45 min and 24 h post-mortem, respectively. Spots that could be identified corresponded mainly to proteins involved in energy-producing pathways (triosephosphate isomerase, enolase, pyruvate dehydrogenase) or to structural proteins (desmin, cap-Z, myosin heavy chain fragment). Persistent under-representation of desmin, a key cytoskeletal protein, in fish submitted to intense muscular activity suggests that such a preslaughter treatment can have an effect on postmortem muscle integrity.

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Dates and versions

hal-02655423 , version 1 (29-05-2020)

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Martine Morzel, Christophe C. Chambon, Florence Lefèvre, Gilles Paboeuf, Elisabeth Laville. Modifications of trout (Oncorhynchus mykiss) muscle proteins by preslaughter activity. Journal of Agricultural and Food Chemistry, 2006, 54, pp.2997-3001. ⟨10.1021/jf0528759⟩. ⟨hal-02655423⟩
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