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Proline-Rich Salivary Proteins Have Extended Conformations

Abstract : Three basic proline-rich salivary proteins have been produced through the recombinant route. IB5 is a small basic proline-rich protein that is involved in the binding of plant tannins in the oral cavity. II-1 is a larger protein with a closely related backbone; it is glycosylated, and it is also able to bind plant tannins. II-1ng has the same polypeptidic backbone as II-1, but it is not glycosylated. Small angle x-ray scattering experiments on dilute solutions of these proteins confirm that they are intrinsically disordered. IB5 and II-1ng can be described through a chain model including a persistence length and cross section. The measured radii of gyration (R(g) = 27.9 and 41.0 +/- 1 angstrom respectively) and largest distances (r(max) = 110 and 155 +/- 10 angstrom respectively) show that their average conformations are rather extended. The length of the statistical segment (twice the persistence length) is b = 30 angstrom, which is larger than the usual value (18 angstrom - 20 angstrom) for unstructured polypeptide chains. These characteristics are presumably related to the presence of polyproline helices within the polypeptidic backbones. For both proteins, the radius of gyration of the chain cross-section is R(c) = 2.7 +/- 0.2 angstrom. The glycosylated protein II-1 has similar conformations but the presence of large polyoside sidegroups yields the structure of a branched macromolecule with the same hydrophobic backbone and hydrophilic branches. It is proposed that the unusually extended conformations of these proteins in solution facilitate the capture of plant tannins in the oral cavity.
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https://hal.inrae.fr/hal-02663056
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Submitted on : Sunday, May 31, 2020 - 1:52:16 AM
Last modification on : Tuesday, September 21, 2021 - 5:02:02 PM

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Helene Boze, Therese Marlin, Dominique Durand Dominique, Javier Perez, Aude Vernhet, et al.. Proline-Rich Salivary Proteins Have Extended Conformations. Biophysical Journal, Biophysical Society, 2010, 99 (2), pp.656-665. ⟨10.1016/j.bpj.2010.04.050⟩. ⟨hal-02663056⟩

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