High fat maternal diet impacts the nutrient sensing signaling network in rabbit blastocyst - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Conference Poster Year : 2016

High fat maternal diet impacts the nutrient sensing signaling network in rabbit blastocyst

Sophie Calderari
Luc Jouneau
Julie Gatien
  • Function : Author
  • PersonId : 937677


The periconceptional period is recognised as a particularly vulnerable period for environmental programming. Maternal nutrition during developmental stages may trigger effects on several physiological functions of the organism. We have previously shown that a maternal lipid and cholesterol-enriched diet (HH diet) administred to rabbit does from prepubertal period and throughout gestation induces fetal growth retardation and components of the metabolic syndrome in adult offspring. HH diet induces lipid droplet accumulation at the blastocyst stage. We aimed to identify the impact of maternal HH diet on rabbit blastocyst gene expression. We performed transcriptomic profiles of blastocysts obtained from rabbit does fed with either a HH diet (n=15) or a control diet (n=12). For this purpose, customized rabbit microarray (Agilent Technologies, GEO platform GPL16709) was used. Statistical analysis (Limma) revealed 49 probes differentially expressed in HH vs control blastocysts (padj<0.05). We were able to annotate 26 genes, 16 up and 10 down-regulated. Functional classification based on GO terms indicated that differentially expressed genes are mainly implicated in lipid transporter activity (RBP4, SCP2) and glucose metabolic process (RBP4, PGD, OMA1). In addition to this approach, we used Gene Set Enrichment Analysis (GSEA) to identify significant enrichment of gene sets (FDR<0.25, p<0.05). Beyond the confirmation of deregulation of glucose and lipid metabolisms, GSEA highlighted enrichment of mTORC1, a master growth regulator that senses growth factors, amino acids, energy status levels to regulate cell growth, proliferation and lipid metabolism. In conclusion, we demonstrated that maternal HH diet altered nutrient sensing and cellular metabolism in blastocyst. These transcriptomic datas will be soon integrated with metabolomic datas obtained on HH and control blastocoelic fluids using ultrasound biomicroscopy puncture by NMR.
Not file

Dates and versions

hal-02741142 , version 1 (03-06-2020)


  • HAL Id : hal-02741142 , version 1
  • PRODINRA : 368859


Sophie Calderari, Catherine Archilla, Luc Jouneau, Nathalie N. Peynot, Nathalie N. Daniel, et al.. High fat maternal diet impacts the nutrient sensing signaling network in rabbit blastocyst. Epiconcept Conference 2016, Sep 2016, Giardini Naxos, Italy. COST Office, 84 p., 2016, Epigenetics and Periconception Environment. ⟨hal-02741142⟩
16 View
0 Download


Gmail Facebook Twitter LinkedIn More