The tomato Guanylate-Binding Protein SlGBP1 enables fruit tissue differentiation by maintaining endopolyploid cells in a non-proliferative state - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Journal Articles The Plant cell Year : 2020

The tomato Guanylate-Binding Protein SlGBP1 enables fruit tissue differentiation by maintaining endopolyploid cells in a non-proliferative state

Constance Musseau
  • Function : Author
  • PersonId : 1075593
Jean-Philippe Mauxion
  • Function : Author
  • PersonId : 1137443
  • IdHAL : jp-mauxion
Isabelle Atienza
  • Function : Author
  • PersonId : 1203874
Christian Chevalier
Christophe Rothan
Frederic Gévaudant

Abstract

Cell fate maintenance is an integral part of plant cell differentiation and the production of functional cells, tissues, and organs. Fleshy fruit development is characterized by the accumulation of water and solutes in the enlarging cells of parenchymatous tissues. In tomato (Solanum lycopersicum), this process is associated with endoreduplication in mesocarp cells. The mechanisms that preserve this developmental program, once initiated, remain unknown. We show here that analysis of a previously identified tomato ethyl methanesulfonate-induced mutant that exhibits abnormal mesocarp cell differentiation could help elucidate determinants of fruit cell fate maintenance. We identified and validated the causal locus through mapping-by-sequencing and gene editing, respectively, and performed metabolic, cellular, and transcriptomic analyses of the mutant phenotype. The data indicate that disruption of the SlGBP1 gene, encoding GUANYLATE BINDING PROTEIN1, induces early termination of endoreduplication followed by late divisions of polyploid mesocarp cells, which consequently acquire the characteristics of young proliferative cells. This study reveals a crucial role of plant GBPs in the control of cell cycle genes, and thus, in cell fate maintenance. We propose that SlGBP1 acts as an inhibitor of cell division, a function conserved with the human hGBP-1 protein.
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Dates and versions

hal-02914290 , version 1 (23-07-2024)

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Constance Musseau, Joana Jorly, Stéphanie Gadin, Iben Sørensen, Catherine Deborde, et al.. The tomato Guanylate-Binding Protein SlGBP1 enables fruit tissue differentiation by maintaining endopolyploid cells in a non-proliferative state. The Plant cell, 2020, 32 (10), pp.3188-3205. ⟨10.1105/tpc.20.00245⟩. ⟨hal-02914290⟩
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