The objective of this study was to evaluate the effect of tree occurrence and density on grass yield, phenological stage (SMP), and nutritional value during the second grassland growth cycle. Three plots with different tree densities (0, 60, and 150 trees/ha) were used. Plots were sampled every 15 days between July 25 and October 27, 2017 The plots did not differ in grass yield, SMP, chemical composition (ie., various measures), or nutritional value. No differences between data sampling were found for yield, smp, and cell wall content. Furthermore, tree occurrence had no effect on grass nutritional value across the second growth cycle.