Ruminant feed utilisation efficiency is determined in part by organic matter digestibility (OMD). However, OMD is expensive and time consuming to measure it in routine. Faecal near infrared (faecal-NIR) spectroscopy has been developed for predicting OMD in cattle across, but its potential for estimating the between-animal variability of OMD has not been reported. The objective of this study was to evaluate the use of of faecal-NIR for assessing the between-animal variability of OMD across different dietary and experimental conditions in cattle. Faecal samples from individual beef and dairy cows associated (n=454) to individual measured OMD values were used. Values ranged from 55.1 and 85.0% (mean±SD; 70.6±4.20%) for 9 experiments at 4 EU sites using 50 different diets. A NIR calibration model for predicting OMD was obtained and validated by cross-validation. Relationship between cross-validated OMD predictions and measured OMD values were analysed by regression analysis with faecal-NIR cross-validated predictions as fixed factor and diet within experiment as random factor. The faecal-NIR prediction was positive and correlated (P<0.001) to measured OMD. The model residual standard error was 2.05%. The effect of diet within experiment was significant (P<0.05) but minor since it representing 25% of error. For a given diet, the correlation coefficients between measured and faecal-NIR predictions when higher when measured OMD variability between animals was high. Our data highlight the potential of faecal-VIS/NIR to discriminate individuals in terms of OMD within a same diet. It could be used as an economical high-throughput tool for exploring between animal variability of OMD.