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Use of the Dynamic TIM-1 Model for an In-Depth Understanding of the Survival and Virulence Gene Expression of Shiga Toxin-Producing Escherichia coli in the Human Stomach and Small Intestine

Abstract : Due to obvious ethical and technical reasons, it remains very difficult to evaluate the survival and expression of virulence genes of food-borne pathogens, such as Shiga toxin-producing Escherichia coli (STEC) in the human gastrointestinal tract. Here, we describe the use of the dynamic TNO (Toegepast Natuurwetenschappelijk Onderzoek) gastrointestinal model (TIM-1) as a powerful in vitro tool to obtain the kinetics of STEC survival by plate counting, the regulation of major virulence genes by RT-qPCR, and the production of Shiga toxins by ELISA, in the human stomach and small intestine. The gut model was adapted in order that in vitro digestions were performed both under adult and child digestive conditions, specific at risk populations for STEC infections. STEC Survival Virulence genes Shiga toxins Human GIT In vitro gut models TIM-1
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https://hal.inrae.fr/hal-03174234
Contributor : Stéphanie Dutilloy <>
Submitted on : Friday, March 19, 2021 - 8:54:37 AM
Last modification on : Saturday, March 20, 2021 - 3:32:00 AM

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Ophélie Uriot, Sandrine Chalancon, Carine Mazal, Lucie Etienne-Mesmin, Sylvain Denis, et al.. Use of the Dynamic TIM-1 Model for an In-Depth Understanding of the Survival and Virulence Gene Expression of Shiga Toxin-Producing Escherichia coli in the Human Stomach and Small Intestine. Methods in Molecular Biology, 2291, In: Schüller S., Bielaszewska M. (eds), pp.297-315, 2021, Shiga Toxin-Producing E. coli, ⟨10.1007/978-1-0716-1339-9_14⟩. ⟨hal-03174234⟩

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