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Exploring the Glucose Fluxotype of the E. coli y-ome Using High-Resolution Fluxomics

Abstract : We have developed a robust workflow to measure high-resolution fluxotypes (metabolic flux phenotypes) for large strain libraries under fully controlled growth conditions. This was achieved by optimizing and automating the whole high-throughput fluxomics process and integrating all relevant software tools. This workflow allowed us to obtain highly detailed maps of carbon fluxes in the central carbon metabolism in a fully automated manner. It was applied to investigate the glucose fluxotypes of 180 Escherichia coli strains deleted for y-genes. Since the products of these y-genes potentially play a role in a variety of metabolic processes, the experiments were designed to be agnostic as to their potential metabolic impact. The obtained data highlight the robustness of E. coli’s central metabolism to y-gene deletion. For two y-genes, deletion resulted in significant changes in carbon and energy fluxes, demonstrating the involvement of the corresponding y-gene products in metabolic function or regulation. This work also introduces novel metrics to measure the actual scope and quality of high-throughput fluxomics investigations.
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https://hal.inrae.fr/hal-03216838
Contributor : Hélène Lesur <>
Submitted on : Tuesday, May 4, 2021 - 1:43:07 PM
Last modification on : Friday, June 11, 2021 - 3:01:26 AM

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metabolites-11-00271-v3.pdf
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Cécilia Bergès, Edern Cahoreau, Pierre Millard, Brice Enjalbert, Mickael Dinclaux, et al.. Exploring the Glucose Fluxotype of the E. coli y-ome Using High-Resolution Fluxomics. Metabolites, MDPI, 2021, 11 (5), pp.271. ⟨10.3390/metabo11050271⟩. ⟨hal-03216838⟩

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