Worldwide Population Structure of Eggplant Identified by SPET Genotyping over 3,400 Accessions - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Conference Papers Year : 2022

Worldwide Population Structure of Eggplant Identified by SPET Genotyping over 3,400 Accessions

Lorenzo Barchi
Ezio Portis
  • Function : Author
Sergio Lanteri
David Alonso
  • Function : Author
Maria José Diez
Jaime Prohens
Hatice Filiz Boyaci
  • Function : Author
Laura Toppino
  • Function : Author
Giuseppe Leonardo
  • Function : Author
Mark Timothy Rabanus-Wallace
  • Function : Author
Andreas Boerner
  • Function : Author
Nils N. Stein
  • Function : Author
Richard Finkers
Matthijs Brouwer
  • Function : Author
Arnaud G. Bovy
  • Function : Author
Roland Schafleitner
  • Function : Author
Giuseppe Aprea
  • Function : Author
Giovanni Giuliano

Abstract

G2P-SOL (http://www.g2p-sol.eu) is an EU-funded project, bringing together the main European and international genebanks hosting germplasm of the four major Solanaceous crops: potato, tomato, pepper and eggplant. Within the project, about 5,900 eggplant accessions, including wild relatives of the crop, have been inventoried. To provide genomic information on around 3,500 representative accessions, a custom set of 5K SPET probes (single primer enrichment technology, TECAN) was designed targeting SNPs evenly distributed all over the genome but mainly in gene-rich regions. DNA samples were prepared by the genebanks, while library preparation and Illumina sequencing was performed by IGA Technology Services. After quality filtering, reads were aligned to the eggplant reference genome sequence (version 4.1) using BWA-MEM and SNP calling was performed using GATK-4.1.9. By removing accessions with low coverage sequencing data, 3,412 were retained and 120K polymorphic sites identified by applying stringent filtering criteria. Among them, 4,306 were SNPs targeted by the 5K probe set, while the remaining were accessory off-target SNPs. The identified SNPs provided information for identifying putative mislabeled accessions (i.e., wrong species assignment), duplicates within and between genebanks. Moreover, they are exploitable for setting up screening protocols to avoid the acquisition of duplications by ex-situ maintainers. The population structure and genetic relationships of the eggplant accessions were investigated to build up a core collection for GWAS. Furthermore, based on historical phenotypic data available in genebanks, loci associated with key agronomic traits were identified. Our results demonstrate that SPET technology represents a valid alternative to random complexity reduction methods and arrays, proving a suitable tool for the high-throughput genotyping, management, and enhancement of genebank collections.

Domains

Genetics
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Dates and versions

hal-03551609 , version 1 (01-02-2022)

Identifiers

  • HAL Id : hal-03551609 , version 1

Cite

Lorenzo Barchi, Ezio Portis, Sergio Lanteri, David Alonso, Maria José Diez, et al.. Worldwide Population Structure of Eggplant Identified by SPET Genotyping over 3,400 Accessions. Virtual Plant and Animal Genome XXIX Conference, Jan 2022, San Diego (CA), United States. ⟨hal-03551609⟩
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