Bacteroides thetaiotaomicron uses a widespread extracellular DNase to promote bile-dependent biofilm formation - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Proceedings of the National Academy of Sciences of the United States of America Year : 2022

Bacteroides thetaiotaomicron uses a widespread extracellular DNase to promote bile-dependent biofilm formation

Abstract

Bacteroides thetaiotaomicron is a gut symbiont that inhabits the mucus layer and adheres to and metabolizes food particles, contributing to gut physiology and maturation. Although adhesion and biofilm formation could be key features for B. thetaiotaomicron stress resistance and gut colonization, little is known about the determinants of B. thetaiotaomicron biofilm formation. We previously showed that the B. thetaiotaomicron reference strain VPI-5482 is a poor in vitro biofilm former. Here, we demonstrated that bile, a gut-relevant environmental cue, triggers the formation of biofilm in many B. thetaiotaomicron isolates and common gut Bacteroidales species. We determined that bile-dependent biofilm formation involves the production of the DNase BT3563 or its homologs, degrading extracellular DNA (eDNA) in several B. thetaiotaomicron strains. Our study therefore shows that, although biofilm matrix eDNA provides a biofilm-promoting scaffold in many studied Firmicutes and Proteobacteria, BT3563-mediated eDNA degradation is required to form B. thetaiotaomicron biofilm in the presence of bile.
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Origin : Publication funded by an institution

Dates and versions

hal-03571822 , version 1 (15-02-2022)

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Attribution - NonCommercial - NoDerivatives

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Nathalie Béchon, Jovana Mihajlovic, Anne-Aurélie Lopes, Sol Vendrell-Fernández, Julien Deschamps, et al.. Bacteroides thetaiotaomicron uses a widespread extracellular DNase to promote bile-dependent biofilm formation. Proceedings of the National Academy of Sciences of the United States of America, 2022, 119 (7), pp.e2111228119. ⟨10.1073/pnas.2111228119⟩. ⟨hal-03571822⟩
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