Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Bioresource Technology Year : 2022

Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles

Christophe Sandt

Abstract

This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes. Infra-red microspectrometry, mass spectrometry, fluorescence recovery after photobleaching and fluorescence imaging were combined to investigate enzymatic hydrolysis at the cell scale. Depending on their polymer composition and organisation, cell types exhibits different extent and rate of enzymatic degradation. Enzymes act sequentially from the cell walls rich in accessible cellulose to the most recalcitrant cells. This phenomenon can be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with the type of cell.

Dates and versions

hal-03664146 , version 1 (10-05-2022)

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Amandine Leroy, M.F. Devaux, Mathieu Fanuel, Hugo Chauvet, Sylvie Durand, et al.. Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles. Bioresource Technology, 2022, 353, pp.1-10. ⟨10.1016/j.biortech.2022.127140⟩. ⟨hal-03664146⟩
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