In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella - Archive ouverte HAL Access content directly
Book Sections Year : 2022

In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella

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Abstract

Applications of bioluminescence for the in vivo study of pathogenic microorganisms are numerous, ranging from the quantification of virulence gene expression to measuring the effect of antimicrobial molecules on the colonization of tissues and organs by the pathogen. Most studies are performed in mice, but recent works demonstrate that this technique is applicable to larger animals like fish, guinea pigs, ferrets, and chickens. Here, we describe the construction and the utilization of a constitutively luminescent strain of Salmonella Typhimurium to monitor in vivo and ex vivo the colonization of mice in the gastroenteritis, typhoid fever, and asymptomatic carriage models of Salmonella infection.
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hal-03699898 , version 1 (20-06-2022)

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Attribution - CC BY 4.0

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Michael Koczerka, Isabelle Lantier, Anne Pinard, Marie Morillon, Justine Deperne, et al.. In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella. Ohad Gal-Mor. Bacterial Virulence : Methods and Protocols, 2427, Humana Press, 2022, Methods in Molecular Biology, 978-1-0716-1970-4. ⟨10.1007/978-1-0716-1971-1_19⟩. ⟨hal-03699898⟩
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