Impact of Environmental Conditions on the Protein Content of Staphylococcus aureus and Its Derived Extracellular Vesicles - Archive ouverte HAL Access content directly
Journal Articles Microorganisms Year : 2022

Impact of Environmental Conditions on the Protein Content of Staphylococcus aureus and Its Derived Extracellular Vesicles

(1, 2) , (1, 2) , (1) , (3) , (1) , (1) , (1) , (2) , (1)
1
2
3
Yves Le Loir
Eric Guédon

Abstract

Staphylococcus aureus, a major opportunistic pathogen in humans, produces extracellular vesicles (EVs) that are involved in cellular communication, the delivery of virulence factors, and modulation of the host immune system response. However, to date, the impact of culture conditions on the physicochemical and functional properties of S. aureus EVs is still largely unexplored. Here, we use a proteomic approach to provide a complete protein characterization of S. aureus HG003, a NCTC8325 derivative strain and its derived EVs under four growth conditions: early- and late-stationary growth phases, and in the absence and presence of a sub-inhibitory concentration of vancomycin. The HG003 EV protein composition in terms of subcellular localization, COG and KEGG categories, as well as their relative abundance are modulated by the environment and differs from that of whole-cell (WC). Moreover, the environmental conditions that were tested had a more pronounced impact on the EV protein composition when compared to the WC, supporting the existence of mechanisms for the selective packing of EV cargo. This study provides the first general picture of the impact of different growth conditions in the proteome of S. aureus EVs and its producing-cells and paves the way for future studies to understand better S. aureus EV production, composition, and roles.
Fichier principal
Vignette du fichier
microorganisms-10-01808.pdf (2.92 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-03780474 , version 1 (19-09-2022)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Brenda Silva Rosa da Luz, Vinícius de Rezende Rodovalho, Aurélie Nicolas, Svetlana Chabelskaya, Julien Jardin, et al.. Impact of Environmental Conditions on the Protein Content of Staphylococcus aureus and Its Derived Extracellular Vesicles. Microorganisms, 2022, 10 (9), pp.1808. ⟨10.3390/microorganisms10091808⟩. ⟨hal-03780474⟩
19 View
1 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More