A five-transgene cassette confers broad-spectrum resistance to a fungal rust pathogen in wheat - Archive ouverte HAL Access content directly
Journal Articles Nature Biotechnology Year : 2021

A five-transgene cassette confers broad-spectrum resistance to a fungal rust pathogen in wheat

(1) , (2) , (1) , (3, 4) , (5) , (5) , (6) , (1) , (1) , (1) , (7) , (7) , (8, 9) , (10) , (11) , (12) , (8) , (8) , (8) , (13, 8) , , , , (8)
1
2
3
4
5
6
7
8
9
10
11
12
13
Ming Luo
  • Function : Author
Liqiong Xie
  • Function : Author
Oadi Matny
Dhara Bhatt
  • Function : Author
Mohammad Hoque
  • Function : Author
Mehran Patpour
Chris Sørensen
Peter Dodds
Burkhard Steuernagel
Brande Wulff
Roger Freedman
  • Function : Author
T. Lynne Reuber
  • Function : Author
Brian Steffenson

Abstract

Combining fungal-resistance genes into a single cassette enables the generation of highly resistant wheat lines. Breeding wheat with durable resistance to the fungal pathogen Puccinia graminis f. sp. tritici (Pgt), a major threat to cereal production, is challenging due to the rapid evolution of pathogen virulence. Increased durability and broad-spectrum resistance can be achieved by introducing more than one resistance gene, but combining numerous unlinked genes by breeding is laborious. Here we generate polygenic Pgt resistance by introducing a transgene cassette of five resistance genes into bread wheat as a single locus and show that at least four of the five genes are functional. These wheat lines are resistant to aggressive and highly virulent Pgt isolates from around the world and show very high levels of resistance in the field. The simple monogenic inheritance of this multigene locus greatly simplifies its use in breeding. However, a new Pgt isolate with virulence to several genes at this locus suggests gene stacks will need strategic deployment to maintain their effectiveness.
Not file

Dates and versions

hal-03828410 , version 1 (25-10-2022)

Identifiers

Cite

Ming Luo, Liqiong Xie, Soma Chakraborty, Aihua Wang, Oadi Matny, et al.. A five-transgene cassette confers broad-spectrum resistance to a fungal rust pathogen in wheat. Nature Biotechnology, 2021, 39 (5), pp.561-566. ⟨10.1038/s41587-020-00770-x⟩. ⟨hal-03828410⟩

Collections

INRAE GAFL
0 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More