Cell walls properties of feeds and their impact on protein digestibility: an in vitro study - Archive ouverte HAL Access content directly
Conference Papers Year : 2022

Cell walls properties of feeds and their impact on protein digestibility: an in vitro study

(1) , (1) , (1)
1

Abstract

Because of climate change and the increase in feed/fuel/food competition, the animal feed industry diversifies the ingredients used to formulate feeds for pigs. Often, those ingredients are rich in dietary fibres (DF), which is known to have a negative influence on nutrient bioaccessibility (i.e. release of nutrients from the plant tissue potentially available for digestion) and digestibility (i.e. the hydrolysis by digestive enzymes) in pigs. However, these effects depend on the amount, nature and organisation of DF within the cell wall. The aim of this work was to determine how DF from different sources affected protein bioaccessibility and proteolysis. We hypothesised that the presence of intact cell walls reduced protein hydrolysis. To test this, we conducted an in vitro study where nine feed ingredients commonly used pig feeding (soybean meal, barley, wheat, two varieties of peas, fava bean, chickpea, maize and sugar beet pulp) were digested using a simulated gastrointestinal digestion model. The amount of proteins recovered after incubation without (bioaccessibility) and with (digestibility) enzymes were measured using the Dumas method. Particle size analysis and microscopy observations were also performed to evaluate cell wall integrity. The differences in particle size, protein bioaccessibility, and proteolysis between the ingredients were analysed by one-way ANOVA using R Studio. Protein bioaccessibility differed significantly between the nine ingredients (P<0.01). Protein hydrolysis values also greatly varied among the ingredients (P<0.01) but did not always follow the same trend as that of bioaccessibility, for instance soybean meal hadisplayed the highest amount of protein digested with 96% and sugar beet pulp the lowest with 50%. The particle size distribution and microscopy images provided some explanations for those differences related to physical barrier, swelling and rupture of the cell walls.

Dates and versions

hal-03881116 , version 1 (01-12-2022)

Identifiers

Cite

Myriam M.-L. Grundy, Jaap J. van Milgen, David Renaudeau. Cell walls properties of feeds and their impact on protein digestibility: an in vitro study. 15. International symposium on digestive physiology of pigs (DPP), May 2022, Rotterdam, Netherlands. pp.145, ⟨10.1016/j.anscip.2022.04.003⟩. ⟨hal-03881116⟩

Collections

INRAE
0 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More