Since 2012, the development of the Crispr-Cas9 system, genome editing (GE) tools have gained a major place in biological research for its simplicity of using. At this moment, the use of this technology is nevertheless not allowed on farm animals, and the application of GE in agriculture is controversial. The objective of this study was to better understand the positions of the actors of the livestock sector with respect to the use of genome editing techniques, that is to say to better understand their arguments and justifications. A qualitative survey by semi-directive interviews was conducted with different actors of the livestock sector. A total of 48 people were interviewed, the respondents belonged to four sectors (ruminants, pigs, poultry and aquaculture). Uncertainties on which the controversy is built are linked to the technology, its impact on the environment and biodiversity, on animals and on its socio-political impact. Our results allowed us to establish a typology of stakeholders in five categories (opponent, sceptical, cautious, enthusiastic, and convinced) and to characterize the main arguments associated with the position of the different stakeholders.