Thermal Behavior of Pea and Egg White Protein Mixtures - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Journal Articles Foods Year : 2023

Thermal Behavior of Pea and Egg White Protein Mixtures

Abstract

The partial substitution of animal protein by plant protein is a new opportunity to produce sustainable food. Hence, to control the heat treatment of a composite protein ingredient, this work investigated the thermal behavior of mixtures of raw egg white (EW) and a laboratory-prepared pea protein isolate (PPI). Ten-percentage-by-weight protein suspensions prepared with different PPI/EW weight ratios (100/0, 75/25, 50/50, 25/75, 0/100) at pH 7.5 and 9.0 were analyzed by differential scanning calorimetry (DSC) and dynamic rheology in temperature sweep mode (T < 100 • C). The DSC data revealed changes in the thermal denaturation temperatures (Td) of ovotransferrin, lysozyme, and pea legumin, supposing interactions between proteins. Denaturation enthalpy (∆H) showed a high pH dependence related to pea protein unfolding in alkaline conditions and solubility loss of some proteins in admixture. Upon temperature sweeps (25-95 • C), the elastic modulus (G) of the mixtures increased significantly with the EW content, indicating that the gel formation was governed by the EW protein. Two thermal sol-gel transitions were found in EW-containing systems. In particular, the first sol-gel transition shifted by approximately +2-3 • C at pH 9.0, probably by a steric hindering effect due to the presence of denatured and non-associated pea globulins at this pH.
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hal-04150556 , version 1 (04-07-2023)

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Jian Kuang, Pascaline Hamon, Valérie Lechevalier, Rémi Saurel. Thermal Behavior of Pea and Egg White Protein Mixtures. Foods, 2023, 12, pp.2528. ⟨10.3390/foods12132528⟩. ⟨hal-04150556⟩
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