Microbiota promotes recruitment and pro-inflammatory response of caecal macrophages during E. tenella infection - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Gut Pathogens Year : 2023

Microbiota promotes recruitment and pro-inflammatory response of caecal macrophages during E. tenella infection

Florian Tomal
Alix Sausset
Yves Le Vern
C. Techer
  • Function : Author
Sonia Lacroix-Lamandé
Fabrice Laurent
Anne Silvestre

Abstract

Background: Eimeria genus belongs to the apicomplexan parasite phylum and is responsible for coccidiosis, an intestinal disease with a major economic impact on poultry production. Eimeria tenella is one of the most virulent species in chickens. In a previous study, we showed a negative impact of caecal microbiota on the physiopathology of this infection. However, the mechanism by which microbiota leads to the physiopathology remained undetermined. Macrophages play a key role in inflammatory processes and their interaction with the microbiota during E. tenella infection have never been investigated. We therefore examined the impact of microbiota on macrophages during E. tenella infection. Macrophages were monitored in caecal tissues by immunofluorescence staining with KUL01 antibody in non-infected and infected germ-free and conventional chickens. Caecal cells were isolated, stained, analyzed and sorted to examine their gene expression using high-throughput qPCR. Results: We demonstrated that microbiota was essential for caecal macrophage recruitment in E. tenella infection. Furthermore, microbiota promoted a pro-inflammatory transcriptomic profile of macrophages characterized by increased gene expression of NOS2, ACOD1, PTGS2, TNFα, IL1β, IL6, IL8L1, IL8L2 and CCL20 in infected chickens. Administration of caecal microbiota from conventional chickens to germ-free infected chickens partially restored macrophage recruitment and response. Conclusions: Taken together, these results suggest that the microbiota enhances the physiopathology of this infection through macrophage recruitment and activation. Consequently, strategies involving modulation of the gut microbiota may lead to attenuation of the macrophage-mediated inflammatory response, thereby limiting the negative clinical outcome of the disease.
Fichier principal
Vignette du fichier
2023_Tomal_Gut-Pathogens_vol-15.pdf (8.19 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Licence : CC BY - Attribution

Dates and versions

hal-04416438 , version 1 (25-01-2024)

Licence

Attribution

Identifiers

Cite

Florian Tomal, Alix Sausset, Yves Le Vern, L. Sedano, C. Techer, et al.. Microbiota promotes recruitment and pro-inflammatory response of caecal macrophages during E. tenella infection. Gut Pathogens, 2023, 15 (1), pp.65. ⟨10.1186/s13099-023-00591-8⟩. ⟨hal-04416438⟩
20 View
4 Download

Altmetric

Share

Gmail Facebook X LinkedIn More