Engineering Yarrowia lipolytica as a Cellulolytic Cell Factory for Production of p-Coumaric Acid from Cellulose and Hemicellulose
Résumé
Denovo biosynthesis of high-value added food additive p-coumaric acid (p-CA) direct from cellulose/hemicellulose is
a more sustainable route compared to the chemical route, considering the abundant cellulose/hemicellulose resources. In this study,
a novel factory was constructed for the production of p-CA in Yarrowia lipolytica using cellulose/hemicellulose as the sole carbon
source. Based on multicopy integration of the TAL gene and reprogramming the shikimic acid pathway, the engineered strain
produced 1035.5 ± 67.8 mg/L p-CA using glucose as a carbon source. The strains with overexpression of cellulases and
hemicellulases produced 84.3 ± 2.4 and 65.3 ± 4.6 mg/L p-CA, using cellulose (carboxymethyl-cellulose) or hemicellulose (xylan
from bagasse) as the carbon source, respectively. This research demonstrated the feasibility of conversion of cost-effective cellulose/
hemicellulose into a value-added product and provided a sustainable cellulolytic cell factory for the utilization of cellulose/
hemicellulose.