Assessment of the performance of the Ames MPF™ assay: A multicenter collaborative study with six coded chemicals - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Journal Articles Mutation Research - Genetic Toxicology and Environmental Mutagenesis Year : 2024

Assessment of the performance of the Ames MPF™ assay: A multicenter collaborative study with six coded chemicals

Dimitrios Spiliotopoulos
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Cécile Koelbert
  • Function : Author
Marc Audebert
Mathilde Constans
  • Function : Author
Francis Finot
  • Function : Author
Laure Khoury
  • Function : Author
Sachiko Kitamoto
  • Function : Author
Laure Malesic
  • Function : Author
Isabelle Mouche
  • Function : Author

Abstract

The Ames MPF™ is a miniaturized, microplate fluctuation format of the Ames test. It is a standardized, commercially available product which can be used to assess mutagenicity in Salmonella and E. coli strains in 384- well plates using a color change-based readout. Several peer-reviewed comparisons of the Ames MPF™ to the Ames test in Petri dishes confirmed its suitability to evaluate the mutagenic potential of a variety of test items. An international multicenter study involving seven laboratories tested six coded chemicals with this assay using five bacterial strains, as recommended by the OECD test guideline 471. The data generated by the participating laboratories was in excellent agreement (93%), and the similarity of their dose response curves, as analyzed with sophisticated statistical approaches further confirmed the suitability of the Ames MPF™ assay as an alternative to the Ames test on agar plates, but with advantages with respect to significantly reduced amount of test substance and S9 requirements, speed, hands-on time and, potentially automation.
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hal-04671419 , version 1 (14-08-2024)

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Dimitrios Spiliotopoulos, Cécile Koelbert, Marc Audebert, Ilona Barisch, Deborah Bellet, et al.. Assessment of the performance of the Ames MPF™ assay: A multicenter collaborative study with six coded chemicals. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 2024, 893, pp.503718. ⟨10.1016/j.mrgentox.2023.503718⟩. ⟨hal-04671419⟩
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