Animal behaviour can be modified in response to stimuli like weather conditions, social structure and farm management. The aim of this study was to determine if routine management activities such as weighing affected cow daily behaviour. Twelve 4 yearold lactating Charolais beef cows received a diet that met 100% of their nutritional requirements during 5 months of lactation. From the second month post-calving, cows underwent feed restriction (4 d to 10 d) during which feed allowance was reduced to meet 50% of their energy requirement (Challenge periods), and then cows returned to full feed (Recovery periods). Cows were equipped with Medria® Axel loggers which recorded physical activity continuously during the study at 5-min intervals, providing the most dominant behaviour among five activities (ingestion, rumination, rest, overactivity and other). Cows were moved from their pen to a scale and weighed at 13:30 on some days. In order to determine the effect of weighing on the patterns of daily behaviour, the same number of days with weighing (BW, n=25 d) and without weighing (W0, n=25 d) were considered for each Challenge and Recovery periods (n=50 d). The main differences during the Challenges were found in the “rumination” time, which was longer in W0 than in BW days, implying that weighing around midday interfered mostly with rumination. Some effects were also observed on “rest” and “over- activity”, while during Recovery periods no differences were found in any activity. These results should be considered in order to schedule routine management to avoid and/or minimize interference with cattle natural behaviour patterns.