In recent years, consumer’s demand for healthy food products have increased considerably. As a result, much research is dedicated to the development of functional foods, i.e. food containing valuable bioactive compounds. Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the human brain and is involved in many physiological mechanisms such as anxiety management or blood pressure lowering. Supplementary GABA intake through food could thus be of interest for consumer’s health. Many microorganisms of food interest have been reported to produce GABA indicating that its content can be increased by microbial fermentation. Indeed, GABA production from L-glutamate is a bacterial mechanism to maintain pH homeostasis. The objective of the present work was thus to identify GABA-producing Lactic acid Bacteria and Propionic Bacteria of food interest, using a simple colorimetric method exploiting this mechanism