The abundance and diversity of Pseudomonas syringae in the Durance river through a watershed in a Mediterranean fruit and vegetable production region - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Conference Poster Year : 2023

The abundance and diversity of Pseudomonas syringae in the Durance river through a watershed in a Mediterranean fruit and vegetable production region

Cindy E. Morris
Odile Berge
Christelle Lacroix
Charlotte Chandeysson
  • Function : Author
  • PersonId : 1203157
Caroline Guilbaud
Cecile Monteil

Abstract

Anticipation of plant disease emergence in the context of global change will require further knowledge on the ecology of plant pathogens, notably for those present in multiple habitats. We have yet to explore factors affecting their spreading, maintenance and diversification (Morris et al. 2017). In stark contrast with human and animal pathogens, little is known about plant pathogens that live outside of agriculture. The P. syringae group of bacteria is an emblematic model plant pathogen, isolated from many substrates, especially in water environments. Monteil et al. (2016) revealed that P. syringae pathogens emerged from freshwater populations, which has led us to focus on aquatic populations. Here, we explored the abundance and diversity of P. syringae populations throughout the Durance-river watershed in the Southern French Alps, as a previous survey revealed they are always present in the headwater creeks. Water collected at 21 sites over a 2-year period resulted in > 5000 putative isolates of P. syringae whose identification was confirmed by partial sequences of the citrate synthase gene. P. syringae was detected in nearly all samples from the fall, winter and spring. In contrast, populations were below the detectable level in 24% of summer samples, almost exclusively in the southern half of the watershed. Population densities ranged from 10^3 to 10^5 cells/L with temperature as a major factor contributing to their variation. One haplotype from phylogroup 2 (mostly including pathogenic strains) was frequently isolated and detected at all sites. Current work aims to reveal the riskiest lines of P. syringae, their origins and trajectories throughout the basin. Morris CE, Barny MA, Berge O, Kinkel LL, Lacroix C (2017). Mol Plant Pathol 18 (2), 308-319. DOI : 10.1111/mpp.12508 Monteil CL, Yahara K, Studholme DJ, Mageiros L, Méric G, Swingle B., Morris CE, Vinatzer BA, Sheppard SK (2016). Microbial Genomics 2 (10). DOI: 10.1099/mgen.0.000089
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hal-04736285 , version 1 (14-10-2024)

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Cindy E. Morris, Odile Berge, Christelle Lacroix, Charlotte Chandeysson, Caroline Guilbaud, et al.. The abundance and diversity of Pseudomonas syringae in the Durance river through a watershed in a Mediterranean fruit and vegetable production region. 16. Rencontres Plantes Bactéries, Mar 2023, Aussois, France. ⟨hal-04736285⟩
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