Ligand discrimination in hOR1A1 based on the capacitive response - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Article Dans Une Revue Biosensors and Bioelectronics Année : 2025

Ligand discrimination in hOR1A1 based on the capacitive response

Résumé

Odorant discrimination mechanisms are based on the differential interactions between odorant molecules and olfactory receptors (ORs). Biohybrid sensors based on ORs described to date show selectivity towards specific versus non-specific binding of odorants, being unable to distinguish between specific ligands of different affinity. Here we disclose a method that enables odorant discrimination based on the modulation of the capacitive response of the receptor, which allows the differentiation of three high-affinity hOR1A1 agonists. We performed voltammetry and impedance measurements of the hOR1A1 receptor selectively immobilized on a gold electrode in the absence and presence of the agonists. Binding induces a decrease in the capacitive response of the receptor that is proportional to the ligand potency, reaching up to a 40% decrease for the cognate ligand dihydrojasmone, which is attributed to changes in the magnitude and orientation of the electric dipole in the receptor, thereby regulating its response to the applied electric field.
Fichier non déposé

Dates et versions

hal-04818964 , version 1 (04-12-2024)

Identifiants

Citer

Anna Lagunas, Christine Belloir, Maxence Lalis, Loïc Briand, Jérémie Topin, et al.. Ligand discrimination in hOR1A1 based on the capacitive response. Biosensors and Bioelectronics, 2025, 271, pp.117000. ⟨10.1016/j.bios.2024.117000⟩. ⟨hal-04818964⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

More