Optimization and validation of a combined iDISCO/CUBIC method for studying global c-fos expression in the mouse brain
Optimisation et validation d'une méthode combinant iDISCO et CUBIC pour étudier l'expression du gène précoce c-fos dans le cerveau de souris
Résumé
Whole-brain imaging following tissue clearing and immunolabeling as offers several advantages over traditional histological methods for revealing networks associated with c-Fos activation. Among the available techniques, iDISCO is considered one of the most effective for immunolabeling and has been widely adopted in recent years. However, the use of post-fixed brain samples remains challenging due to several limitations—most notably, tissue autofluorescence in microvessels and partial damage to the olfactory bulbs. In order to reveal neuronal network activated after odor exposure, we aimed to establish a tissue clearing method for post-fixed brain samples, to optimize it for studying neural networks of the olfactory system, and to set up an analysis pipeline.
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