Bovine mastitis (BM) is a major disease in the dairy industry, which negatively impacts the milk quality. In a previous study, we highlighted that a post-milking application of a Lacticaseibacillus paracasei (Lp1542) strain on the bovine teat skin is safe with regard to mammary gland functionality and integrity while having an impact on its microbiota. The present study aims to further evaluate the safety of such strategy by assessing how the presence of this strain in milk can impact the downstream dairy processes. Firstly, the heat sensitivity of Lp1542 was demonstrated for representative milk heat treatment conditions. Secondly, Lp1542 does not interfere with the acidification capabilities of a commercial starter culture nor its proteolytic activities. In conclusion, the combined results highlighted that a topical treatment with Lp1542 is safe with regard to animal health as well as downstream dairy processes inviting us to further explore its effectiveness for BM prevention.