An in vitro alternative to the in sacco method to determine ruminal starch degradation - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Conference Poster Year : 2024

An in vitro alternative to the in sacco method to determine ruminal starch degradation

Abstract

Starch is a pivotal constituent of many ruminant feeds, serving as a key energy source. Determining its degradation in the rumen is essential, as it enables to calculate the risk of acidosis and indirectly determines the partitioning of energy digestion between the rumen and the intestine, thereby influencing nutrients intake. The part of degradable starch in the rumen used to be determined on animals with the in sacco method. However, societal demand on animal welfare led to the development of in vitro methods to determine ruminal degradation of feeds, recognizing as well the significant cost and time savings offered by laboratory methods. An enzymatic method was developed to estimate starch ruminal degradation and tested on 17 common concentrated feed used in ruminant feeding (cereals, by-products, feed mixtures and legume seeds). Starch content of feed varied from 197 to 682 g/kg DM. The method consists of incubating dried and ground samples, at 60°C in buffer pH6 for one hour with a Bacillus sp. alpha-amylase (10069, Sigma-Aldrich, France). Enzymatic starch degradation was calculated as the ratio between the quantities of starch degraded (recovered from the residue after incubation) and the initial starch content of feed. This enzymatic in vitro starch degradation was compared to the in sacco ruminal degradation (assuming passage rate of 6%/h) of starch (ED6_St). ED6_St varied from 64% to 97% and enzymatic degradation varied from 39 to 96%. The coefficient of determination between the enzymatic method and the ED6_St was 69.9%, and the residual standard deviation was 6.3%. These first results are promising to obtain an in vitro method to assess ruminal starch degradation. However, further tests are required to complete and validate the method, including the incorporation of a broader array of feed types, such as forages.
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Dates and versions

hal-04651215 , version 1 (17-07-2024)

Identifiers

  • HAL Id : hal-04651215 , version 1

Cite

Maria Arias-Benavides, Remy Delagarde, Pierre Noziere, Gaëlle Maxin. An in vitro alternative to the in sacco method to determine ruminal starch degradation. 75. Annual meeting of the european federation of animal science (EAAP), Sep 2024, Florence, Italy. Wageningen Academic Publishers, Annual Meeting of the European Association for Animal production, 34, pp.849, 2024, Book of abstracts of the 75th annual meeting of the european federation of animal science. ⟨hal-04651215⟩
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