Optimization of permeabilized fibers preparation for mitochondrial respiration measurements using Design of Experiments methodology.
Résumé
To optimize the permeabilized fibers (pf) preparation from mouse Tibialis anterior in our lab, we used the Design of Experiments (DoE) methodology that
evaluates the impact of 6 experimental conditions or factors, on the pf respiration parameters (Pyruvate Malate Succinate respiration (PMS leak) and
respiratory control ratio (RCRPMS)), to provide a maximum of information using a limited number of experiments and animals.
Domaines
Sciences du Vivant [q-bio]
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2017_Chabi_Poster COST MITOEAGLE 2017 final.pdf (829.17 Ko)
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