Rationale: Microbiota composition of patients with Short Bowel Syndrome (SBS) is characterized by a high prevalence of Lactobacillus. Transferring this microbiota to germ-free rats induced certain adaptations observed in SBS patients, such as colonic hyperplasia. In this study, 2 bacterial strains isolated from this SBS-microbiota were used to test their individual effects on the setting of adaptations in a rat model of SBS. Methods: Wistar rats underwent 80% resection of the small intestine, ileocecal valve and right colon or intestinal transection and received a daily supplementation with either strain A or B for 28 days. Body weight, food intake and faecal appearance were measured daily. Faeces and blood were collected for microbiome and gastrointestinal hormone analyses respectively. Digestive transit time was evaluated using carmine red. Jejunum and colon were sampled for morphometric analyses. Results are expressed as mean ±SEM and statistical analyses used non parametric tests (2-way and 1-way ANOVA, Kruskal-Wallis test). Results: Supplementation with the 2 strains had no impact on microbiota diversity and composition compared with untreated SBS rats. All SBS rats lost weight the 1rst week and then regained some, with no difference between SBS groups. SBS (p=0.0201) and SBS+A (p=0.0207) rats exhibited a significant increase of food intake compared to SHAM rats whereas SBS+B rats did not (p=0.7249). At D28, SBS treated rats had less diarrhoea and a slowed transit time compared to untreated SBS rats. The thickness of the jejunal muscular layer was significantly increased in the SBStreated groups (p<0,001). Conclusion: This study indicates a beneficial effect of these probiotics since supplementation with strain A or B led to diarrhoea improvement, suggesting a reduction in water loss. Furthermore, the treatment helped slowing down the transit time, allowing longer contact between food and the absorption surface.